Measurement of Glucose in Human Blood Serum Using Near - Infrared Raman Spectroscopy

This thesis discusses the development of a near infrared spontaneous Raman scattering spectroscopic system designed to analyze blood chemical composition in human blood serum. Several analytic methods have been studied and verified for analyzing the Raman spectrum. Three experiments have been performed with a step by step approach to isolate and solve problems. Using this Raman spectroscopic system, we measured the glucose concentration level in phosphate buffered saline with an average error of 4 mg/dL while the reference error is 2.5 mg/dL. In single donor serum, the average prediction error is 8 mg/dL while the reference error is 2.5 mg/dL. In multidonor human blood serum the average error is 18 mg/dL while the reference error is 8 mg/dL. Other blood analytes such as albumin, total protein, cholesterol and triglyceride have also been measured. Thesis Supervisor: Michael S. Feld Title: Professor of Physics